Fiorentin TR, Krotulski AJ
Society of Forensic Toxicology- 2020 Webinar ConferenceSynthetic cannabinoids continue to appear in forensic toxicology casework, creating high risk scenarios for drug users as newer generations can be more potent than previous iterations. Synthetic cannabinoids represent a chemically diverse class of substances with particularly difficult behaviors to which high resolution mass spectrometry (HRMS) can provide great benefit.
To better understand its toxicity and implication in forensic casework, a quantitative confirmatory test was developed and validated for 5F-MDMB-PICA (the most prevalent synthetic cannabinoid identified in forensic casework) and its metabolites in human blood. A Thermo Scientific™ Vanquish UHPLC Systems coupled with a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer was used for analysis. Chromatographic separation was achieved using a reverse phase gradient across a Thermo Scientific™ Accucore™ Vanquish™ C18+ analytical column (100 x 2.1mm, 2.6µm). The method was quantitatively validated in accordance with ASB Standard Practices for Method Validation in Forensic Toxicology.
Data was processed using Thermo Scientific™ TraceFinder™ Clinical software (Version 4.1). This interactive presentation will provide an overview of Thermo Scientific™ software, specifically with respect to its use in determining method setpoints and building a library database. Performance characteristics will be reviewed to demonstrate the utility and accuracy of the developed assay.
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